Recombinant Rat Ctss protein(Met1-Ile318), His-tagged

• Cat.No.: Ctss-1113R
• Product Overview: Recombinant Rat Ctss (Met1-Ile318) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-318 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVENval-WRK (Dnp)-NH2. The specific activity is >300 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)
• Molecular Mass: The recombinant rat Ctss consists 329 amino acids and predicts a molecular mass of 37.3 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ctss cathepsin S [ Rattus norvegicus ]
• Official Symbol: Ctss
• Synonyms: CTSS; cathepsin S
• Gene ID: 50654
• mRNA Refseq: NM_017320
• Protein Refseq: NP_059016