| Species : |
Rat |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
54-202 aa |
| Form : |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
Endothelins (EDN) are small (21 amino acids) vasoactive peptides producedbymany cell types including endothelial and epithelial cells, macrophages and fibroblasts. By binding to G-protein-linked transmembrane receptors, EDNsparticipate in vasoconstriction modulation and cell growth regulation. It hasbeenproven that EDN1 has chemotaxis active on monocytes, thus chemotaxisassayused 24-well microchemotaxis system was undertaken to detect the chemotacticeffect of EDN1 on the human monocytic cell line THP-1. Briefly, THP-1cellswereseeded into the upper chambers (100uL cell suspension, 106cells/mLinRPMI 1640 with 0.5%FBS) and EDN1 (20ng/mL, 40ng/mL and 80ng/mLdilutedseparately in serum free RPMI 1640) was added in lower chamber withapolycarbonate filter (8µm pore size) used to separate the two compartments. Afterincubation at 37°C with 5%CO2 for 3h, the filter was removed, then cellsinlowchamber were observed by inverted microscope at low magnification (×100) andthe number of migrated cells were counted at high magnification (×400) randomly(five fields for each filter). Result shows EDN1 is able to induce migrationof THP-1cells. The migrated THP-1 cells in low chamber at low magnification (×100) wereshown. Five fields of each chamber were randomly chosen, andthemigrated cells were counted at high magnification (×400). Statistical resultswereshown. The optimum chemotaxis of EDN1 occurs at 80ng/mL. |
| Molecular Mass : |
18.5kDa |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
