Active Recombinant Rat Enpp3 protein, His-tagged, Low Endotoxin

• Cat.No.: ENPP3-1762R
• Product Overview: Recombinant Rat Enpp3 protein(P97675)(46-875 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 46-875 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 15000 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 95.37 kDa. Due to glycosylation, the protein migrates to 115-135 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Enpp3 ectonucleotide pyrophosphatase/phosphodiesterase 3 [ Rattus norvegicus ]
• Official Symbol: Enpp3
• Synonyms: ENPP3; ectonucleotide pyrophosphatase/phosphodiesterase 3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; B10; E-NPP 3; PD-Ibeta; RB13-6 antigen; phosphodiesterase I beta; alkaline phosphodiesterase; phosphodiesterase I/nucleotide pyrophosphatase 3; Npp3; RB13-6
• Gene ID: 54410
• mRNA Refseq: NM_019370
• Protein Refseq: NP_062243