Recombinant Rat Gadd45a Protein, His-tagged

• Cat.No.: Gadd45a-1558R
• Product Overview: Recombinant Rat Gadd45a Protein (Met1-Arg165) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable several functions, including promoter-specific chromatin binding activity; protein N-terminus binding activity; and protein dimerization activity. Involved in G2/M transition of mitotic cell cycle. Predicted to be located in nuclear speck. Predicted to be active in cytoplasm and nucleus. Orthologous to human GADD45A (growth arrest and DNA damage inducible alpha).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.2 kDa
  Accurate Molecular Mass: 22 kDa
• Protein length: Met1-Arg165
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gadd45a growth arrest and DNA-damage-inducible, alpha [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gadd45a
• Synonyms: GADD45A; growth arrest and DNA-damage-inducible, alpha; growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible protein GADD45 alpha; Ddit1; Gadd45
• Gene ID: 25112
• mRNA Refseq: NM_024127
• Protein Refseq: NP_077041
• UniProt ID: P48317