Active Recombinant Rat Gadd45a protein, His-tagged

• Cat.No.: Gadd45a-1558R
• Product Overview: Active Recombinant Rat Gadd45a protein(P48317)(1-165 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 1-165 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45a) is a member of the GADD45 family. It may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Besides, Proliferating Cell Nuclear Antigen (PCNA) has been identified as an interactor of GADD45a, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GADD45a and recombinant rat PCNA. Briefly, GADD45a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to PCNA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GADD45a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of GADD45a and PCNA was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 22.2kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gadd45a growth arrest and DNA-damage-inducible, alpha [ Rattus norvegicus ]
• Official Symbol: Gadd45a
• Synonyms: GADD45A; growth arrest and DNA-damage-inducible, alpha; growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible protein GADD45 alpha; Ddit1; Gadd45
• Gene ID: 25112
• mRNA Refseq: NM_024127
• Protein Refseq: NP_077041