Recombinant Rat Gck protein, His & GST-tagged

• Cat.No.: Gck-749R
• Product Overview: Recombinant Rat Gck aa. (Asp194~Gly410 (Accession # P17712)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Asp194~Gly410
• Description: Catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 54.1kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 4.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Gck glucokinase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gck
• Synonyms: GLK; GLUKA; RNGK2; HK IV; HK4; glucokinase variant 3; hexokinase type IV; hexokinase-4; hexokinase-D; glucokinase
• Gene ID: 24385
• mRNA Refseq: NM_001270849.1
• Protein Refseq: NP_001257778.1
• UniProt ID: P17712