Recombinant Rat Gria2 Protein, His-tagged

• Cat.No.: Gria2-1588R
• Product Overview: Recombinant Rat Gria2 Protein (Asn413-Lys527) with N-His tag was expressed in E. coli.

Specification

• Description: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.5 kDa
  Accurate Molecular Mass: 16 kDa
• Protein length: Asn413-Lys527
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gria2 glutamate receptor, ionotropic, AMPA 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gria2
• Synonyms: GRIA2; glutamate receptor, ionotropic, AMPA 2; glutamate receptor 2; glutamate receptor B; AMPA-selective glutamate receptor 2; GluA2; GluR2; gluR-B; GluR-K2
• Gene ID: 29627
• mRNA Refseq: NM_001083811
• Protein Refseq: NP_001077280
• UniProt ID: P19491