Recombinant Rat IFNA1 protein(Met1-Ser192), hFc-tagged

• Cat.No.: IFNA1-2349R
• Product Overview: Recombinant Rat IFNA1 (P05011) (Met 1-Ser 192) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ser192
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 1-10 μg/mL.
• Molecular Mass: The recombinant rat IFNA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 410 amino acids and predicts a molecular mass of 46.4 kDa. The apparent molecular mass of the rat IFNA1/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ifna1 interferon-alpha 1 [ Rattus norvegicus ]
• Official Symbol: IFNA1
• Synonyms: IFNA1; interferon-alpha 1; interferon alpha-1; IFN-alpha1
• Gene ID: 298210
• mRNA Refseq: NM_001014786
• Protein Refseq: NP_001014786