Recombinant Rat Nox3 protein, His-tagged

• Cat.No.: Nox3-7662R
• Product Overview: Recombinant Rat Nox3 aa. (Gly221~Gln466) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly221~Gln466
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 6.4.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Nox3 NADPH oxidase 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Nox3
• Synonyms: NADPH oxidase 3
• Gene ID: 292279
• mRNA Refseq: NM_001004216.1
• Protein Refseq: NP_001004216.1
• UniProt ID: Q672K1