Recombinant Rat Npnt Protein, His-tagged

• Cat.No.: Npnt-2502R
• Product Overview: Recombinant Rat Npnt Protein (Ile302-Cys558) with a N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable integrin binding activity. Predicted to be involved in several processes, including cell-cell adhesion mediated by integrin; pilomotor reflex; and positive regulation of alkaline phosphatase activity. Predicted to act upstream of or within several processes, including branching involved in ureteric bud morphogenesis; positive regulation of cell-substrate adhesion; and positive regulation of transforming growth factor beta receptor signaling pathway. Predicted to be located in basement membrane; extracellular region; and smooth muscle contractile fiber. Orthologous to human NPNT (nephronectin).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.6 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Ile302-Cys558
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Npnt nephronectin [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Npnt
• Synonyms: Npnt; nephronectin; nephronectin
• Gene ID: 686753
• mRNA Refseq: NM_001402176
• Protein Refseq: NP_001389105
• UniProt ID: A0A0G2K8C1