Recombinant Rat Vascular Endothelial Growth Factor C, His-tagged

• Cat.No.: Vegfc-635R
• Product Overview: Recombinant ratVegfc, lacking the N- and C-terminal extensions and containing only themiddle VEGF homology domain, forms primarily non-covalently linked dimers.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: His
• Description: Vegfc, also knownas Vascular Endothelial Growth Factor Related Protein (VRP), is a recentlydiscovered VEGF growth factor family member that is most closely related toVEGF-D. The rat Vegfc cDNA encodes a pre-pro-protein of 416 amino acidsresidues.
• Form: lyophilized
• Purity: > 90%, bySDS-PAGE and visualized by silver stain.
• Molecular Weight: 36-48 kDa
• Range: 100-400 ng/ml
• Endotoxin Level: < 0.1 ng per μgof Vegfc152S
• Biological Activity: 5 x 103units/mg. Measured by its ability to stimulate phosphorylation of theVEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells).The ED50 for this effect is typically 150-300 ng/ml.
• Stabilizer: BSA
• Reconstitution: Centrifuge vialbriefly before opening to bring contents to bottom of vial. The lyophilized Vegfc152Sis soluble in water and most aqueous buffers. The lyophilized Vegfc152Sshould be reconstituted in PBS or medium to a concentration not lower than 50μg/ml.
• Storage: Lyophilized samplesare stable for greater than six months at -20°C to -70°C. Reconstituted Vegfc152Sshould be stored in working aliquots at -20°C. Avoid repeated freeze-thawcycles.
• OfficialSymbol: Vegfc
• Pathways: Bladder cancer; Cytokine-cytokinereceptor interaction; Focal Adhesion; Focal adhesion; Hemostasis; Pancreaticcancer; Pathways in cancer; Platelet activation; Platelet degranulation; Renalcell carcinoma; Response to elevated platelet cytosolic Ca2+; SignalTransduction; Signaling by VEGF; VEGF binds to VEGFR leading to receptordimerization; mTOR signaling pathway

Gene Information

• Gene Name: Vegfc vascular endothelialgrowth factor C [ Rattus norvegicus ]
• Synonyms: Vegfc;vascular endothelial growth factor C; VRP; VEGF-C; flt4-L;flt4 ligand; vascular endothelial growth factor-related protein; Vascular endothelial growth factor C; Flt4 ligand; Vascular endothelial growth factor-related protein
• Gene ID: 114111
• mRNA Refseq: NM_053653
• Protein Refseq: NP_446105
• UniProt ID: O35757
• Chromosome Location: 16p11
• Function: chemoattractantactivity; growth factor activity; vascular endothelial growth factor receptor3 binding