Recombinant RSV G Protein, His-tagged

• Cat.No.: G-475V
• Product Overview: Recombinant RSV G Protein(O36633-1) (His 67-Ala 299) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 67-299 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant human RSV strain B1 glycoprotein G comprises 244 amino acids with a predicted molecular mass of 27 kDa. As a result of highly glycosylation, the apparent molecular mass of the recombinant protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.