Recombinant S. aureus Sortase A Protein, His tagged

• Cat.No.: SAOUHSC_02834-46S
• Product Overview: Sortase A from Staphylcoccus aureus was expressed in E. coli expression system with a N-terminal His tag. The activity of Sortase A can be measured in a FRET-based enzymatic assay

Specification

• Species: S.aureus
• Source: E.coli
• Tag: His
• Protein Length: 26-206aa
• Description: Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since Sortases are widely distributed among a variety of bacterial pathogens and required for virulence, Sortases represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.
• Tag: N-His
• Form: Solution
• Molecular Mass: 21.7 kDa
• Applications: FRET; Protease activity
• Usage: Research use
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/ml
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, and 2.2 mM KCl, 8 mM imidazole, 0.04% Tween 20, and 20 % glycerol.
• References: 1. Spirig T, et al. Mol Microbiol 82, 1044 (2011).
  2. Marraffini LA, et al. Microbiol Mol Biol Rev 70, 192 (2006).
  3. Maresso AW, et al. Pharmacol Rev 60, 128 (2008).
  4. Proft T. Biotechnol Lett 32, 1 (2010).

Gene Information

• Gene Name: SAOUHSC_02834 sortase [ Staphylococcus aureus subsp. aureus NCTC 8325 ]
• Official Symbol: SAOUHSC_02834
• Synonyms: SAOUHSC_02834; sortase; sortase, putative; Sortase A
• Gene ID: 3921273
• Protein Refseq: YP_501293
• UniProt ID: Q2FV99