Recombinant S. pyogenes Cas9 Protein, His-tagged
Cat.No. : | Cas9-03S |
Product Overview : | Recombinant full-length S. pyogenes Cas9 was expressed in E. coli with a C-terminal His tag. |
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Description : | S. pyogenes Cas9 is a type II-A Cas9 protein and is the most used in most current CRISPR-based genetic engineering methodologies. CRIPSR RNAs (crRNAs) fused to trans-activating CRISPR RNAs (tracrRNA) direct sequence-specific cleavage by Cas9, introducing double-stranded breaks in cell-invading DNA in bacterial and archaeal CRIPSR/Cas9 systems. Cas9 requires the 5'-NGG-3' protospacer adjacent motif (PAM) to destabilize the target DNA. Cas9's HNH endonuclease domain cleaves the strand complementary to the crRNA while its RuvC endonuclease domain cleaves the non-complementary strand. |
Source : | E. coli |
Species : | S. pyogenes |
Tag : | His |
Molecular Mass : | ~156 kDa |
Purity : | >80% |
Stability : | One year at -70 centigrade from deta of shipment. |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.5 μg/μL 6.26 pmol/μg |
Storage Buffer : | Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol. |
Shipping : | On dry ice. |
Official Symbol : | Cas9 |
Synonyms : | Cas9; Csn1; Spy_1046 |
UniProt ID : | X78809 |
Official Symbol : | Cas9 |
Synonyms : | Cas9; Csn1; Spy_1046 |
UniProt ID : | X78809 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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