Recombinant S. pyogenes Cas9 Protein, His-tagged

• Cat.No.: Cas9-03S
• Product Overview: Recombinant full-length S. pyogenes Cas9 was expressed in E. coli with a C-terminal His tag.

Specification

• Species: S.pyogenes
• Source: E.coli
• Tag: His
• Description: S. pyogenes Cas9 is a type II-A Cas9 protein and is the most used in most current CRISPR-based genetic engineering methodologies. CRIPSR RNAs (crRNAs) fused to trans-activating CRISPR RNAs (tracrRNA) direct sequence-specific cleavage by Cas9, introducing double-stranded breaks in cell-invading DNA in bacterial and archaeal CRIPSR/Cas9 systems. Cas9 requires the 5'-NGG-3' protospacer adjacent motif (PAM) to destabilize the target DNA. Cas9's HNH endonuclease domain cleaves the strand complementary to the crRNA while its RuvC endonuclease domain cleaves the non-complementary strand.
• Molecular Mass: ~156 kDa
• Purity: >80%
• Stability: One year at -70 centigrade from deta of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.5 μg/μL
  6.26 pmol/μg
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.
• Shipping: On dry ice.

Gene Information

• Official Symbol: Cas9
• Synonyms: Cas9; Csn1; Spy_1046
• UniProt ID: X78809