| Species : |
Salmon/Trout |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Recombinant salmon (Oncorhynchus kisutch)/ trout (O.mykiss) insulin-like growth factor-I (rsIGF-I) is a 70 amino acid polypeptide. IGF-I stimulates growth and differentiation in most cell types. IGF-I mediates many of the anabolic properties of growth hormone. |
| Molecular Weight : |
Theoretical: 7704 daltons. By Mass Spectrometry: 7719 daltons. Amino acid sequence and total amino acid analysis confirmed that the recombinant product is salmon/trout IGF-I. |
| N-terminal Sequence Analysis Of Recombinant Product : |
5 residues >95% single sequence. |
| Purity : |
> 95% (as determined by HPLC and N-terminal sequence analysis). |
| Appearance : |
White Powder. |
| Biological Activity : |
Stimulation of protein synthesis in L6 myoblasts -ED50 32.5 ng/ml. |
| Endotoxin (LAL) : |
< 0.1 EU/ug. |
| Reconstitution : |
Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.< 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. |
| State : |
Lyophilised from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa). |
| Stability : |
At least 2 years at 2-4°C (lyophilised). |
