Recombinant SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) Protein, His-tagged

• Cat.No.: S-466S
• Product Overview: Recombinant SARS-CoV-2 (2019-nCoV) Spike S1+S2 (YP_009724390.1, with mutations L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) (Met1-Pro1213) was expressed in insect cell with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.

Specification

• Source: Insect cell
• Species: SARS-CoV-2
• Tag: His
• Predicted N Terminal: Val 16
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike S1+S2 consists of 1209 amino acids and predicts a molecular mass of 134.4 kDa.
• Protein length: 1-1213
• Endotoxin: < 1.0 EU/μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
  Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
  Please contact us for any concerns or special requirements.
  Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• mRNA Refseq: MN908947
• Protein Refseq: YP_009724390