Active Recombinant SARS-CoV-2 Spike RBD (L452R, E484Q) Protein, His/AVI-tagged, Biotinylated
| Cat.No. : | S-402S |
| Product Overview : | Recombinant SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452R, E484Q) (Arg319-Phe541) was expressed in HEK293 cell with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617, B.1.617.1, B.1.617.3) which emerged in the India. |
- Specification
- Gene Information
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| Source : | HEK293 |
| Species : | SARS-CoV-2 |
| Tag : | His/AVI |
| Predicted N Terminal : | Arg 319 |
| Bio-activity : | Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R, E484Q) Protein (His/AVI Tag), Biotinylated, the EC50 of SARS-CoV-2 Spike RBD (L452R, E484Q) Protein (His/AVI Tag), Biotinylated is 10-60 ng/mL. |
| Molecular Mass : | The recombinant SARS-CoV-2 Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.4 kDa. As a result of glycosylation, it migrates as an approximately 35.88 kDa band in SDS-PAGE under reducing conditions. |
| Protein length : | 319-541 |
| Endotoxin : | < 1.0 EU/μg protein as determined by the LAL method. |
| Purity : | > 95 % as determined by SDS-PAGE. |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. |
| Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
| Official Symbol : | S |
| Synonyms : | S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein |
| Gene ID : | 43740568 |
| mRNA Refseq : | MN908947 |
| Protein Refseq : | YP_009724390 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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