Recombinant SARS-CoV Spike protein, His-tagged, Biotinylated

• Cat.No.: Spike-0373S
• Product Overview: Biotinylated Recombinant SARS-CoV Spike protein(Met1-Arg667)(AAX16192.1), fused with His tag, was expressed in Insect Cells.

Specification

• Species: SARS-CoV
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Arg667
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant S1 subunit of SARS-CoV (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.