Recombinant SARS-CoV Spike S2 protein(Ser668-Pro1195), His-tagged

• Cat.No.: Spike-1230V
• Product Overview: Recombinant SARS-CoV spike S2 (NP_828851.1) (Ser668-Pro1195) was expressed in Insect Cells with a C-terminal polyhistidine tag.

Specification

• Source: Insect Cells
• Species: SARS
• Tag: His
• Protein length: Ser668-Pro1195
• Form: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV spike S2 consists of 539 amino acids and predicts a molecular mass of 59.65 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.