Recombinant SARS S, His-tagged

• Cat.No.: S-390S
• Product Overview: A DNA sequence encoding the receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: SARS
• Source: Insect Cells
• Tag: His
• Protein Length: Arg306-Phe527
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike comprises 233 amino acids and has a predicted molecular mass of 26.5 kDa. The apparent molecular mass of the protein is approximately 35.1 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: S E2 glycoprotein precursor [ SARS coronavirus ]
• Official Symbol: S
• Synonyms: S; E2 glycoprotein precursor; spike glycoprotein; NP_828851.1; As established by Krokhin et al. (2003), the glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
• Gene ID: 1489668
• Protein Refseq: NP_828851