Recombinant Simian Immunodeficiency Virus VPX Protein (1-112 aa), His-SUMO-tagged

• Cat.No.: VPX-882S
• Product Overview: Recombinant Simian Immunodeficiency Virus (isolate K78) (SIV-mac) VPX Protein (1-112 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.
• Source: E. coli
• Species: Simian Immunodeficiency Virus
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 29.2 kDa
• Protein length: 1-112 aa
• AA Sequence: MSDPRERIPPRNSGEETIGEAFEWL NRTVEEINREAVNHLPRELIFQVWQ RSWEYWHDEQRMSQSYVKYRYLCLM QKALFMHCKKGCRCLGEGHRAGGWR PGPPPPPPPGLA
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.
• UniProt ID: P11266

Gene Information

• UniProt ID: P11266