Recombinant Staphylococcus aureus Protein A protein, His-tagged

• Cat.No.: Protein A-3149S
• Product Overview: Recombinant Staphylococcus aureus Protein A protein(P02976), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Staphylococcus aureus
• Source: E.coli
• Tag: His
• Form: Lyophilized from additive free solution.
• Specificity: The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
• Molecular Mass: Approximately 34.8 kDa with 6×His-tag and a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 307 amino acids.
• AASequence: MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC
• Endotoxin: Less than 0.1 EU/μg of Protein A-Cys, His as determined by LAL method.
• Purity: > 96 % by SDS-PAGE and RP-HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Concentration: 4.589 mg/ml
• Reconstitution: Dissolve in distilled water or saline.