Recombinant Streptococcus pyogenes cas9 protein

• Cat.No.: cas9-11S
• Product Overview: Recombinant Streptococcus pyogenes Cas9 was expressed in E. coli.

Specification

• Species: Streptococcus pyogenes
• Source: E.coli
• Tag: Non
• Protein Length: 2-1368 aa
• Form: 30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4.
• Bio-activity: The in vitro cleavage activity of the three batches of Cas9 Nuclease was basically the same.
• Molecular Mass: The protein has a predicted MW of 163 kDa.
• Endotoxin: ≤10 EU/mg
• Purity: ≥95% by Bis-Tris PAGE; ≥95% by SEC-HPLC.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20 ± 5°C.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.