Recombinant Streptococcus Pyogenes CRISPR-Cas9 Protein, His tagged

• Cat.No.: CRISPR-Cas9-12S
• Product Overview: The Streptococcus Pyogenes CRISPR-Cas9 Protein, His Tag is produced in Insect Cells. A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.

Specification

• Species: Streptococcus Pyogenes
• Source: Insect Cells
• Tag: His
• Protein Length: 1-1368 aa
• Molecular Mass: 163 kDa
• Purification: Affinity purification
• Endotoxin: <1.0 EU/μg determined by the LAL method
• Purity: > 90% determined by SDS-PAGE
• Storage: Aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 20mM Tris, 300mM NaCl, 50% Glycerol, 0.5mM PMSF, 0.1mM EDTA, 0.5mM TCEP, pH8.0
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

Gene Information

• Synonyms: CRISPR-Cas9
• Protein Refseq: WP_010922251