Active Recombinant Streptococcus sp. Streptokinase G Protein

• Cat.No.: skg-1378S
• Product Overview: Active Recombinant Streptococcus sp. Streptokinase G Protein(P10519)(27-440 aa) was expressed in E. coli.

Specification

• Species: Streptococcus
• Source: E.coli
• Tag: Non
• Protein Length: 27-440 aa
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 0.02% Tween20.
• Bio-activity: Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8.0 × 10^4 IU/mg.
• Molecular Mass: Approximately 47.3 kDa, a single non-glycosylated polypeptide chain containing 414 amino acids.
• AASequence: IAGPEWLLDR PSVNNSQLVV SVAGTVEGTN QDISLKFFEI DLTSRPAHGG KTEQGLSPKS KLFATDSGAM PHKLEKADLL KAIQEQLIAN VHSNDDYFEV IDFASDATIT DRNGKVYFAD KDGSVTLPIQ PVQEFLLKGH VRVRPYKEKP VQNQAKSVDV EYTVQFTPLN PDDDFRPALK DTKLLKTLAI GDTITSQELL AQAQSILNKN HPGYTIYERD SSIVTHDNDI FRTILPMDQE FTYHVKNREQ AYRINKKSGL NEEINNTDLI SEKYYVLKKG EKPYDPFDRS HLKLFTIKYV DVNTNELLKS EQLLTASERN LDFRDLYDPR DKAKLLYNNL DAFGIMDYTL TGKVEDNHDD TNRIITVYMG KRPEGENASY HLAYDKDRYT EEEREVYSYL RYTGTPIPDN PNDK
• Endotoxin: Less than 1 EU/μg of rSK as determined by LAL method.
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.