Recombinant Streptomyces coelicolor A3(2) ANMK protein, His-tagged

• Cat.No.: ANMK-980S
• Product Overview: Recombiant Streptomyces coelicolor A3(2) ANMK full length or partial length protein was expressed.

Specification

• Source: E. coli or Yeast
• Species: Streptomyces coelicolor A3(2)
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80% by SDS-PAGE
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage buffer: PBS buffer

Gene Information

• Gene Name: anmK anhydro-N-acetylmuramic acid kinase [ Streptomyces coelicolor A3(2)]
• Official Symbol: ANMK
• Synonyms: ANMK; anhydro-N-acetylmuramic acid kinase; ydhH
• Gene ID: 1103033
• Protein Refseq: NP_631637
• UniProt ID: Q9EWI9

Reviews

07/23/2021

This unique capability to selectively bind to exposed PS simplifies the identification and quantification of different cellular populations, reducing experimental errors and enhancing accuracy.

05/25/2021

They can provide technical assistance, ensuring optimal utilization of the product.

07/26/2017

the manufacturer can contribute to the research process by providing top-quality Annexin-5 protein that meets rigorous standards of purity, stability, and reliability.

05/11/2016

This may include providing detailed protocols, troubleshooting guidance, and access to knowledgeable experts who can answer any inquiries or concerns.

Q&As

Is ANMK expressed in all cell types?

ANMK expression has been observed in various cell types, including both proliferating and non-dividing cells. However, its expression levels and functions may differ among cell types and tissues.

Are there any drugs or therapeutic targets that aim to modulate ANMK activity?

Currently, there are no specific drugs or therapeutic targets that directly modulate ANMK activity. However, as ANMK regulates the activity of Aurora and NIMA kinases, which have been implicated in cancer, drugs targeting these kinases indirectly affect ANMK-mediated pathways. For example, several Aurora kinase inhibitors, such as alisertib and danusertib, have been developed and are being investigated in clinical trials for cancer treatment.

Are there any known interactions or binding partners of ANMK?

ANMK is known to directly interact and activate Aurora and NIMA kinases. Additionally, studies have suggested interactions between ANMK and other proteins involved in cell division, such as spindle assembly factors and mitotic checkpoint proteins. Further research is needed to fully elucidate all the binding partners and interactions of ANMK within the cell.

How does ANMK activate Aurora and NIMA kinases?

ANMK directly interacts with and activates Aurora and NIMA kinases by binding to their catalytic domains. This interaction stimulates the kinase activity of these enzymes, allowing them to phosphorylate and regulate downstream target proteins involved in cell division processes.

Is ANMK expressed in all tissues or is it tissue-specific?

There is limited information available regarding the tissue expression profile of ANMK. According to existing data, ANMK is expressed in multiple tissues, including the brain, heart, liver, lung, and kidney, suggesting a widespread distribution throughout the body. However, further studies are necessary to determine the exact tissue-specific expression pattern of ANMK and to explore its potential functional significance in different tissues.

Are there any diseases associated with ANMK dysregulation?

Currently, there is limited information about diseases directly linked to ANMK dysregulation. However, dysregulation or mutations in the Aurora and NIMA kinases, which ANMK activates, have been implicated in various human diseases, including cancer and developmental disorders.

What are the biological consequences of ANMK activation?

ANMK activation leads to the phosphorylation of specific substrates by Aurora and NIMA kinases. This phosphorylation cascade regulates mitotic events, including spindle formation, chromosome dynamics, and cytokinesis. Proper activation of these kinases by ANMK ensures accurate and efficient cell division.

Can ANMK be used as a biomarker for disease diagnosis or prognosis?

The potential use of ANMK as a biomarker for disease diagnosis or prognosis is not well-established. However, some studies have suggested that increased ANMK expression could be associated with poor prognosis in certain types of cancer. Further research is needed to validate ANMK as a reliable biomarker and to determine its clinical relevance in various diseases.

Can ANMK mutations lead to genetic diseases?

As of now, there is no known evidence linking ANMK mutations to any specific genetic diseases. However, it is important to note that the function and implications of ANMK in various biological processes are still being examined. Future studies may reveal associations between ANMK mutations and specific genetic diseases or disorders.

Is ANMK involved in any other cellular processes apart from cell division?

While ANMK's primary role is in regulating cell division processes, there is emerging evidence suggesting its involvement in other cellular processes as well. Some studies have suggested a potential role for ANMK in DNA damage response, cell migration, and neuronal development. However, further research is needed to fully understand the extent of ANMK's involvement in these processes.

Are there any known diseases or disorders associated with ANMK dysfunction?

Currently, there are no specific diseases or disorders directly linked to ANMK dysfunction. However, dysregulation of the kinases it activates, such as Aurora kinases, has been associated with various conditions including cancer, neurodegenerative diseases, and developmental disorders. ANMK-mediated pathways may play a role in these diseases, but further research is needed to clarify the extent of ANMK's involvement.

Can ANMK be targeted for therapeutic purposes?

Targeting ANMK directly might be challenging due to its involvement in critical cellular processes such as cell division. However, targeting downstream kinases activated by ANMK, such as Aurora and NIMA kinases, has been explored as a potential therapeutic strategy for certain diseases, particularly cancer.

Are there any known inhibitors or activators of ANMK?

As of now, there is limited knowledge about specific inhibitors or activators of ANMK. Further research is needed to identify small molecules or signaling pathways that regulate ANMK activity.

Are there any known mutations or genetic variations in the ANMK gene?

Currently, there is limited information available about specific mutations or genetic variations in the ANMK gene. Genetic variations, such as single-nucleotide polymorphisms (SNPs), may exist in the ANMK gene, but their functional significance and association with disease have yet to be fully explored.