Recombinant Vaccinia Virus B18R protein, His-tagged

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Recombinant Vaccinia Virus B18R protein, His-tagged

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Cat.No. : B18R-641V
Product Overview : Recombinant Vaccinia Virus B18R (His20-Glu351) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : B18R is a type I interferon (IFN)-binding protein and it is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus, which contains two Ig-like C2-type domains and one Ig-like V-type domain. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R counteracts the antiviral effects of host IFN-alpha/beta. Also, B18R acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.
Source : HEK293
Species : Vaccinia Virus
Tag : His
Predicted N Terminal : His20
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : His20-Glu351
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of B18R-641V :

Vaccinia Virus B18R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of B18R-641V :

Immobilized Human IFN-α1a at 2 μg/mL (100 μL/well) can bind Vaccinia Virus B18R, His Tag with a linear range of 0.8-6 ng/mL (QC tested).

Gene Name : B18R
Official Symbol : B18R
Synonyms : VACWR200
Gene ID : 3707576
Protein Refseq : YP_233081.1
UniProt ID : P25213

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