Recombinant Vibrio cholerae Cholera Toxin

• Cat.No.: VC1456-102B
• Product Overview: Recombinant Vibrio cholerae Cholera Toxin(P01556)(22-124 aa) was expressed in E.coli.

Specification

• Species: Vibrio cholerae
• Source: E.coli
• Tag: Non
• Protein Length: 22-124 aa
• Form: Supplied as a 0.2 μm filtered solution in 5 mM PB, pH 7.0, 75 mM NaCl, with 50 % glycerol.
• Molecular Mass: Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 103 amino acids.
• AASequence: TPQNITDLCA EYHNTQIYTL NDKIFSYTES LAGKREMAII TFKNGAIFQV EVPGSQHIDS QKKAIERMKD TLRIAYLTEA KVEKLCVWNN KTPHAIAAIS MAN
• Endotoxin: Less than 0.1 EU/µg of rCTB as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.