Recombinant Zebrafish ACTB2

• Cat.No.: ACTB2-9628Z
• Product Overview: Recombinant Zebrafish ACTB2 full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: actb2 actin, beta 2 [ Danio rerio (zebrafish) ]
• Official Symbol: ACTB2
• Gene ID: 57935
• mRNA Refseq: NM_181601
• Protein Refseq: NP_853632
• UniProt ID: A8WG05

Reviews

07/24/2022

The product's contribution to my findings was substantial, allowing me to make meaningful conclusions and discoveries

05/18/2020

I appreciated the company's efforts to create a community of scientists, facilitating knowledge exchange and collaboration

04/05/2020

I was impressed by the company's commitment to innovation, reflected in the product's novel features and capabilities

06/16/2019

I obtained highly reproducible data using the product, enhancing the reliability and robustness of my findings

06/25/2016

I relied on the product's trusted name and positive reviews from fellow researchers, and it did not disappoint.

Q&As

What was the characteristic degradation pattern of GAPDH2 and ACTB2 after death?

GAPDH2 and ACTB2 mRNAs degraded rapidly after death, making them suitable for estimating early PMI.

Did the decline in transcript levels of GAPDH2 and ACTB2 vary based on the reference markers used?

 No, the decline in transcript levels of GAPDH2 and ACTB2 was observed regardless of whether the reference markers were miRs or GAPDH1 and ACTB1.

What can be concluded about the transcript levels of GAPDH2 and ACTB2 in relation to PMI estimation?

The findings suggest that the transcript levels of GAPDH2 and ACTB2 show rapid decline after death and are suitable markers for estimating early PMI.

How did the error rates of GAPDH1 and ACTB1 compare to GAPDH2 and ACTB2?

 After validation, the error rates of GAPDH1 and ACTB1 were much higher than those of GAPDH2 and ACTB2.

Were miRs suitable as reference markers for estimating PMI?

Yes, the error rates of GAPDH2/miRs and ACTB2/miRs were significantly lower than that of GAPDH2/GAPDH1 and ACTB2/ACTB1, confirming the suitability of miRs as reference markers.

Which markers were found to be more appropriate for estimating early PMI?

GAPDH2 and ACTB2, particularly located at the 5'-end of mRNAs, were considered more suitable for estimating early PMI.

What was the advantage of using short genes like GAPDH and ACTB for estimating early PMI?

Short genes like GAPDH and ACTB were found to be more appropriate for estimating early PMI, indicating their suitability for early time points after death.

How was the gene actb2 found to perform in the liver when exposed to different concentrations of cadmium and subsequently infected with Aeromonas hydrophila?

The results obtained by Lang et al. (2016) indicated that actb2 was the most suitable RG in the liver when exposed to different concentration groups of cadmium and subsequently infected with Aeromonas hydrophila.

In the current study, what was observed regarding the stability and suitability of actb2 genes in the intestine and muscle?

The current study found that actb2 genes were more stable and suitable for the intestine and muscle.

What is the relationship between the transcript levels of GAPDH2 and ACTB2 and postmortem interval (PMI)?

The transcript levels of GAPDH2 and ACTB2 rapidly declined in correlation with PMI.