| Cat.No.: | Kit-2372 |
| Product Overview: | Activation of caspases plays a central role in apoptosis. The Red Active Caspase-3 Staining Kit provides a convenient means for detecting activated caspase-3 in; Living cells. The assay utilizes the caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. |
| Applications: | Detection of activated caspase-3 in living cells. |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Kit Components: | Red-VAD-FMK; Wash Buffer; Z-VAD-FMK |
| Target Species: | Mammalian |
| Detection method: | Fluorescence microscopy, fluorescence plate reader(Ex/Em = 540/570 nm) and Flow cytometry |
| Features & Benefits: | Simple one-step procedure; takes only 1-2 hours; Fast and convenient; The red fluorescence label allows for direct detection of activated caspases in apoptotic cells |
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