||Activation of caspases plays a central role in apoptosis. The Red Active Caspase-3 Staining Kit provides a convenient means for detecting activated caspase-3 in; Living cells. The assay utilizes the caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.
||Detection of activated caspase-3 in living cells.
||Red-VAD-FMK; Wash Buffer; Z-VAD-FMK
||Fluorescence microscopy, fluorescence plate reader(Ex/Em = 540/570 nm) and Flow cytometry
|Features & Benefits:
||Simple one-step procedure; takes only 1-2 hours; Fast and convenient; The red fluorescence label allows for direct detection of activated caspases in apoptotic cells