||Activation of caspases plays a central role in apoptosis. The Red Active Caspase-9 Staining Kit provides a convenient means for detecting activated caspase-9 in; Living cells. The assay utilizes a caspase-9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.
||Detection of activated caspase-9 in living cells.
||Red-LEHD-FMK; Wash Buffer; Z-VAD-FMK
||Fluorescence microscopy, fluorescence plate reader(Ex/Em = 540/570 nm) and Flow cytometry
|Features & Benefits:
||Simple one-step procedure; takes only 1-2 hours; Fast and convenient; The fluorescence label allows detection of activated caspase-8 in apoptotic cells