RFP Magnetic CoIP Agarose kit

• Cat.No.: RFP-04A
• Product Overview: The RFP-Trap Magnetic Agarose Kit contains RFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of RFP-fusion proteins and their interacting factors.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Ready-to-use
  • No heavy & light antibody chains on the SDS-PAGE
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
• Binding capacity: 22.5 μg of recombinant RFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 μm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 5 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Upon receipt store at +4°C. Do not freeze!