SIRT1 Activity Assay Kit (Fluorometric)

• Cat.No.: KITZ-001
• Product Overview: SIRT1 Activity Assay Kit (Fluorometric) detects SIRT1 activity in lysates. Primarily, the SIRT1 Activity Assay Kit is designed for the rapid and sensitive evaluation of SIRT1 inhibitors or activators using crude SIRT1 fraction or purified SIRT1. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1 activity with the SIRT1 Activity Assay Kit if the appropriate antibody directed against SIRT1 (Anti-SIRT1 antibody) is used for immunoprecipitation. The kit has been shown to detect the activity of Sirtuins, at least SIRT1 in Human or animal cell lysates or in column fractions. The assay shows good linearity of sample response. The assay may be used to follow the purification of Sirtuins or may be used to detect the presence of Sirtuins in cell lysates.

Specification

• Applications: 1.Monitoring the purification of SIRT1.
  2.Screening inhibitors or activators of SIRT1.
  3.Detecting the effects of pharmacological agents on SIRT1.
• Storage: Store the developer and recombinant SIRT1 at -80°C, all other kit reagents should be stored below -20°C.