|Assay Protocol :
||The method involves adding a media pouch and Escherichiacoli tablet to a 100 mL water sample and incubating for five hours at 39°C. After incubation for five hours, the culture is transferred to fluorescent media and incubated three additional hours. If the culture fluoresces, it is over 90% predictive of coliphage presence and fecal contamination. The 5-hour culture is also spotted on to a prepared, seeded agar plate of E.coli for overnight confirmation and verification of plaque formation, 16 hours from the beginning of testing.