"SMase" Related Products

Sphingomyelinase Activity Fluorometric Assay Kit

Cat.No.: Kit-2279
Product Overview: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 μU/ml in a variety of samples.
Applications: Measurement of SMase activity in various tissues/cells extracts.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: SMase Assay Buffer; SMase Probe (DMSO); SMase Substrate (Lyophilized); SMase Enzyme Mix I (Lyophilized); SMase Enzyme Mix II (Lyophilized); Choline Standard (Lyophilized); SMase Positive Control (Lyophilized); SMase Extraction Detergent
Detection method: Fluorescence (Ex/Em535/587 nm)
Features & Benefits: Simple, rapid & convenient;
The assay can detect sphingomyleinase activity as low as 10 μU/ml in a variety of samples.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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