PDGFB
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Official Full Name
platelet-derived growth factor beta polypeptide
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Overview
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013] -
Synonyms
PDGFB; platelet-derived growth factor beta polypeptide; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog);
- Recombinant Proteins
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- Cell & Tissue Lysates
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- Bovine
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- Mammalian cells
- P. pastoris
- S.cerevisiae
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- C
- His
- GST
- His (Fc)
- Avi
- His|GST
- His|S
- SUMO
- N/A
- N
- mFc
- Tag Free
- Background
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- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- PDGFB Related Articles
What is PDGFB protein?
PDGFB (platelet derived growth factor subunit B) gene is a protein coding gene which situated on the long arm of chromosome 22 at locus 22q13. The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. The PDGFB protein is consisted of 241 amino acids and its molecular mass is approximately 27.3 kDa.
What is the function of PDGFB protein?
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells.
PDGFB Related Signaling Pathway
By binding to its receptor PDGFR, PDGFB activates downstream signaling pathways, including PI3K/Akt, MAPK/ERK and other pathways, thereby promoting biological processes such as cell proliferation, differentiation and migration. At the same time, PDGFB can activate NF-κB signaling pathway, TGF-β signaling pathway and inhibit Wnt/β-catenin signaling pathway, so as to regulate the biological behavior of cells.
PDGFB Related Diseases
Mutations in this gene are associated with meningioma, gastrointestinal stromal tumors(GISTs), osteosarcoma and other tumors. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. PDGFB is involved in nervous system development and repair, and is associated with neurological diseases such as white matter disease and multiple sclerosis.
Fig1. Possible mechanism of PDGFB pathogenesis in Ankylosing spondylitis (AS).
Bioapplications of PDGFB
As a therapeutic target, PDGFB can assist in the development of inhibitor drugs for related diseases. Several applications of PDGFB have been studied or are undergoing clinical trials.
High Purity
Fig1. SDS-PAGE (PDGFB-528H) (PROTOCOL for western blot)
High Bioactivity
Fig2. Activity Data. (PDGFB-4346HG)
Case study 1: Weixin Meng, 2018
Thoracic aortic dissection (TAD) is a serious condition requiring urgent treatment to avoid catastrophic consequences. The inflammatory response is involved in the occurrence and development of TAD. This study aimed to determine whether expression of PDGF-B (a subunit of PDGF-BB) was increased in TAD patients. Full-thickness ascending aorta wall specimens from TAD patients (n = 15) and control patients (n = 10) were examined for expression of PDGF-B and its receptor (PDGFRB) and in terms of morphology, inflammation, and fibrosis. An increase in elastic fragments in the aorta wall might be responsible for inducing the activation and migration of macrophages to injured sites, leading to elevated expression of PDGF-B.
Fig1. Expression of platelet-derived growth factor B (PDGF-B) in ascending aorta wall.
Fig2. Expression levels of PDGF-B protein were significantly elevated in the outer DM in thoracic aortic dissection (TAD) patients compared with control aorta wall, as indicated by Western blot.
Case study 2: Zhicheng Zhang, 2024
Colon adenocarcinoma (COAD) is the most common malignancy of the digestive tract, which is characterized by a dismal prognosis. No effective treatment has been established presently, thus there is an urgent need to understand the mechanisms driving COAD progression. By constructing stable cell lines (KLF7 overexpression and knockdown) and using a series of cellular and molecular experiments, this study found that KLF7 is overexpressed in COAD tissues. Mechanistically, these findings reveal that KLF7 can specifically bind to the promoter region of PDGFB (TGGGTGGAG), thus promoting the transcription of PDGFB and increasing its secretion. And secreted PDGFB facilitates the progression of COAD by activating MAPK/ERK, PI3K/AKT, and JAK/STAT3 signaling pathways through PDGFRβ.
Fig3. Effects of PDGFB in KLF7 transient knockdown or control LoVo cells were determined by migration.
Fig4. KLF7 promotes COAD progression by activating the MAPK/ERK, PI3K/AKT, and JAK/STAT3 signaling pathways through PDGFB.
Fig1. Schematic model illustrating KLF7 promoting COAD progression. KLF7 is overexpressed in COAD and can bind to the promoter region of PDGFB to promote its secretion. (Zhicheng Zhang, 2024)
Fig2. Schematic illustration of the potential mechanism of gastric cancer with liver metastasis (GCLM). (Chuanfu Ren, 2023)
PDGFB involved in several pathways and played different roles in them. We selected most pathways PDGFB participated on our site, such as MAPK signaling pathway, Ras signaling pathway, Rap signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with PDGFB were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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MAPK signaling pathway | Fasl;FGF1B;CACNG6A;TNFRSF1A;FGF6B;MAP3K13;PTPN7;RAC2;PPP5C |
Ras signaling pathway | NF1;PGF;INS1;FGF8;PAK6;PDGFRB;MAPK10;PIK3R3;PLA2G4D |
Rap signaling pathway | INS1;FGF4;FGF22;LPAR2;GNAI1;PDGFC;LAT;ITGAM;CALM2 |
Cytokine-cytokine receptor interaction | CCL15;CXCR4A;PF4V1;PF4;CCL11;OSMR;GHRA;CCL16;TNFB |
Phospholipase D signaling pathway | GRM1;SHC1;PLCB4;PDGFRB;PLCB1;CYTH4;PDGFA;PLD1;GRB2 |
PIK-Akt signaling pathway | PDGFD;IL7R;ITGA3;FGF8;ITGA10;RXRA;YWHAZ;GNG11;PIK3CD |
Focal adhesion | ITGB1A;SHC3;PIK3R5;VTNB;PTK6;PIK3R2;ACTN1;MYL2B;COL5A1 |
Gap junction | GNA11A;TUBA8L4;MAP3K2;TUBA1L2;HRASB;MAP2K2;TUBA3C;PLCB4;PLCB1 |
Regulation of actin cytoskeleton | MYLK4;FGF13B;WASLB;FGF20B;FGFR1A;FGFR3;ROCK2A;PIK3R3;ITGA9 |
HTLV-I infection | WNT11;LCK;DVL2;IL1R1;BAX;TBPL2;ATF3;SRF;CD3D |
Pathways in cancer | EGLN1;GNG2;FGF10;TRAF1;NCOA4;PPARD;TRAF2;ITGAV;FGF8 |
MicroRNAs in cancer | TNN;CCND2;PLCG2;DNMT3A;IRS2;WNT3A;IRS1;SIRT1;ABCC1 |
Renal cell carcinoma | RAPGEF1;MAP2K2;PIK3CD;MAPK3;PIK3R5;RAC1;EP300;MAPK1;MAP2K1 |
Glioma | ARAF;PDGFB;CALM4;CALM1;SHC1;PRKCA;MAP2K2;CAMK2A;PDGFRA |
Prostate cancer | FOXA1;PDGFA;PIK3CD;BRAF;PDPK1;TCF7L2;BAD;SMARCA4;SPINK1 |
Melanoma | AKT2;BRAF;PDGFRA;Fgf15;MAPK3;PIK3CG;TRP53;NRAS;FGF18 |
Choline metabolism in cancer | LYPLA1;RPS6KB1;PDGFC;PDGFD;MAP2K1;DGKB;TAE1;RPS6KB2;PRKCA |
PDGFB has several biochemical functions, for example, chemoattractant activity, collagen binding, growth factor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PDGFB itself. We selected most functions PDGFB had, and list some proteins which have the same functions with PDGFB. You can find most of the proteins on our site.
Function | Related Protein |
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chemoattractant activity | CXCL12A;HMGB2;VEGFC;LGALS3;SAA4;SAA3;MIF;HMGB1;CCL16 |
collagen binding | ANTXR1;CTSK;ADAM9;PAK1;SRGN;ACHE;CD44;SMAD4;GP5 |
growth factor activity | VEGFAB;IL5;FIGF;GPI;Il2;FGF18;AREG;VEGFA;MANF |
identical protein binding | PRDX1;CLDN17;ECI1;LCP1;DDX58;VIM;SERPINA1E;FAM108A1;CLDND |
platelet-derived growth factor binding | COL1A2;COL4A1;COL5A1;PDGFRA;COL3A1;PDGFRB;Pdgfa&Pdgfb;COL1A1;COL2A1 |
platelet-derived growth factor receptor binding | FIGF;PTPRJ;PDGFRB;PDGFA;PDGFD;PDGFB;PTEN;PDGFAA;ITGA5 |
protein binding | MAPK11;TRIM55;ASCC1;MBD4;CDX4;CHUK;DNM1;FAM50B;TUBB2B |
protein heterodimerization activity | CEBPG;HIST1H3C;TLR1;TAS1R1;CUL3;SYT16;MEF2C;PIK3R1;BNIP3 |
protein homodimerization activity | BOK;NR0B2;NAGA;PLCB1;UGT1A4;USH2A;BMP2B;BOKA;ABP1 |
superoxide-generating NADPH oxidase activator activity | NOXA1;PDGFB;AGT;NCF4;NOXO1 |
PDGFB has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PDGFB here. Most of them are supplied by our site. Hope this information will be useful for your research of PDGFB.
PDGFRB; PDGFRA
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Q&As (7)
Ask a questionBy potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.
PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.
PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.
Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.
PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.
Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.
PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.
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