Recombinant Human PDGFBB

• Cat.No.: PDGFB-59H
• Product Overview: Recombinant Human Platelet-Derived Growth Factor BB is a homodimeric, glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 32,021 Dalton. PDGF-BB is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: S.Cerevisiae
• Tag: Non
• Description: PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from 0.2 µm filtered solution in 20mM sodium acetate in absence of any carrier protein.
• Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg.
• Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.
• Storage: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution PDGF BB should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ]
• Synonyms: PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; PDGF subunit B; Platelet-derived growth factor B chain; Platelet-derived growth factor beta; PDGF-2; Platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor 2
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• UniProt ID: P01127
• Chromosome Location: 22q12.3-q13.1; 22q13.1
• MIM: 190040
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by PDGF
• Function: cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; contributes_to platelet-derived growth factor receptor binding; protein heterodimerization activity