Active Recombinant Mouse Pdgfb protein, His-tagged

• Cat.No.: PDGFB-12568M
• Product Overview: Recombinant Mouse Pdgfb protein(Ser82-Thr190), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 82-190 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mMNaAc-Hac pH4.5
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.Immobilized Human PDGFB at 2 μg/mL (100 μL/well) can bind Human PDGFRB with a linear range of 12-153 ng/mL. 2. Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.36-1.42 ng/mL, corresponding to a specific activity of 7.04×10^5~2.78×10^6 units/mg.
• Molecular Mass: 13.06 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Pdgfb platelet derived growth factor, B polypeptide [ Mus musculus ]
• Official Symbol: Pdgfb
• Synonyms: PDGFB; platelet derived growth factor, B polypeptide; platelet-derived growth factor subunit B; c-sis; PDGF-2; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide; Sis; PDGF-B
• Gene ID: 18591
• mRNA Refseq: NM_011057
• Protein Refseq: NP_035187