Active Recombinant Human PDGFB Protein, Pre-aliquoted

• Cat.No.: PDGFB-14H
• Product Overview: Recombinant Human PDGFB Protein, Ser82 - Thr190, was expressed in E. coli.

Specification

• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1.5-6.0 ng/mL.
• Molecular Mass: Predicted Molecular Mass:12.3 kDa (monomer)
  SDS-PAGE: 12 kDa, reducing conditions
• Protein length: Ser82-Thr190
• N-terminal Sequence Analysis: Ser82
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile 4 mM HCl, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: PDGFB platelet derived growth factor subunit B [ Homo sapiens (human) ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet derived growth factor subunit B; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; epididymis secretory sperm binding protein; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127