Recombinant Human S100A8 Protein, His-tagged

• Cat.No.: S100A8-2670H
• Product Overview: Recombinant Human S100A8 Protein (Met1-Glu93) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 12.1 kDa
  Accurate Molecular Mass: 12 kDa
• Protein length: Met1-Glu93
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens (human) ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; CAGA, CFAG, S100 calcium binding protein A8 (calgranulin A), S100 calcium binding protein A8 (calgranulin A); protein S100-A8; 60B8AG; CGLA; MRP8; P8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); MIF; NIF; CAGA; CFAG; L1Ag; CP-10; MA387
• Gene ID: 6279
• mRNA Refseq: NM_002964
• Protein Refseq: NP_002955
• MIM: 123885
• UniProt ID: P05109

Reviews

02/01/2022

Dependable protein quantification, ensures experimental accuracy.

10/29/2020

Consistent western blot results, a lab essential.

03/12/2020

Expert protein engineering support, essential for our projects.

Q&As

How do alterations in S100A8 expression impact cellular stress responses and aging?

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis?

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms?

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes?

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases?

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases?

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders?

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.