Recombinant Human S100A8 Protein (Leu2-Glu93), N-His-tagged

• Cat.No.: S100A8-12H
• Product Overview: Recombinant Human S100A8 (Leu2-Glu93) protein with a N-His tag was derived from the transfected human HEK293 cells.

Specification

• Description: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized powder
• Molecular Mass: 11 kDa
• Protein length: Leu2-Glu93
• Purity: > 95%, SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
• Applications: Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
• Usage: This product is furnished for LABORATORY RESEARCH USE ONLY.
  Not for diagnostic or therapeutic use.
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
• Shipping: Ice packs

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens (human) ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100-A8; protein S100-A8; calgranulin A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; urinary stone protein band A
• Gene ID: 6279
• mRNA Refseq: NM_002964
• Protein Refseq: NP_002955
• MIM: 123885
• UniProt ID: P05109

Reviews

02/01/2022

Dependable protein quantification, ensures experimental accuracy.

10/29/2020

Consistent western blot results, a lab essential.

03/12/2020

Expert protein engineering support, essential for our projects.

Q&As

How do alterations in S100A8 expression impact cellular stress responses and aging?

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis?

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms?

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes?

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases?

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases?

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders?

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.