Active Recombinant Human S100A8 protein, His-tagged

• Cat.No.: S100A8-2216H
• Product Overview: Active Recombinant Human S100A8 protein(NP_002955.2) (Met 1-Glu 93) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-93 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
• Bio-activity: Measured by its ability to bind recombinant human S100A9 in a functional ELISA.
• Molecular Mass: The recombinant human S100A8 consists of 103 amino acids and predicts a molecular mass of 12.2 kDa. It migrates as an approximately 14.6 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_002955.2
• Unit ID: P05109
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P05109
• Publications:
  • Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction. (2016)
  • Myeloid-related protein 8 induces self-tolerance and cross-tolerance to bacterial infection via TLR4-and TLR2-mediated signal pathways. (2015)

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; CAGA, CFAG, S100 calcium binding protein A8 (calgranulin A), S100 calcium binding protein A8 (calgranulin A); protein S100-A8; 60B8AG; CGLA; MRP8; P8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); MIF; NIF; CAGA; CFAG; L1Ag; CP-10; MA387
• Gene ID: 6279
• mRNA Refseq: NM_002964
• protein Refseq: NP_002955
• MIM: 123885