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Recombinant Mouse S100a8 overexpression lysate

Cat.No. : S100a8-12HCL
Product Overview : Recombinant mouse S100a8 HEK293T cell transient overexpression lysate (as WB positive control) with a C-Myc/DDK tag was expressed in HEK293T.
Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Components: 1 vial of 20 μg lyophilized gene specific transient over-expression cell lysate
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Description : S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 10.7 kDa
Stability : Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles after reconstitution.
Storage : Upon receiving, store the sample at -20 centigrade.
Reconstitution : Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Shipping : The lysate can be shipped at ambient temperature.
Gene Name : S100a8 S100 calcium binding protein A8 (calgranulin A) [ Mus musculus (house mouse) ]
Official Symbol : S100a8
Synonyms : S100a8; S100 calcium binding protein A8 (calgranulin A); p; Ca; MR; p8; B8A; CFA; B8Ag; CFAg; CP-1; Caga; MRP8; 60B8A; CP-10; 60B8Ag; AI323541; protein S100-A8; Names; MRP-8; S100 calcium-binding protein A8; calgranulin-A; chemotactic S100 protein; chemotactic cytokine CP-10; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; pro-inflammatory S100 cytokine
Gene ID : 20201
mRNA Refseq : NM_013650
Protein Refseq : NP_038678
UniProt ID : P27005

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do alterations in S100A8 expression impact cellular stress responses and aging? 03/03/2022

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis? 12/12/2021

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms? 03/02/2021

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes? 01/18/2021

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases? 11/01/2019

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases? 09/22/2019

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders? 10/06/2018

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.

Customer Reviews (3)

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Reviews
02/01/2022

    Dependable protein quantification, ensures experimental accuracy.

    10/29/2020

      Consistent western blot results, a lab essential.

      03/12/2020

        Expert protein engineering support, essential for our projects.

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