Recombinant Mouse S100a8, His-tagged

• Cat.No.: S100a8-3545M
• Product Overview: Recombinant mouse S100A8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: S100a8-3545M
• Description: S100A8, also known MRP8, is a member of the S100 family, EF-hand superfamily of calcium binding proteins. This protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis.
• Source: E. coli.
• Formulation: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
• Molecular weight: 12.4 kDa (109aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: S100a8 S100 calcium binding protein A8 (calgranulin A) [ Mus musculus ]
• Synonyms: S100a8; S100 calcium binding protein A8(calgranulinA); p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; A1323541; protein S100-A8;MRP-8; calqranulin-A; chemotactic cytokine CP-10; pro-inflammatory S100 cytokine; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8
• Gene ID: 20201
• mRNA Refseq: NM_013650
• Protein Refseq: NP_038678
• UniProt ID: P27005
• Chromosome Location: 3 F1-F2; 3 43.6 cM
• Function: calcium ion binding

Reviews

02/01/2022

Dependable protein quantification, ensures experimental accuracy.

10/29/2020

Consistent western blot results, a lab essential.

03/12/2020

Expert protein engineering support, essential for our projects.

Q&As

How do alterations in S100A8 expression impact cellular stress responses and aging?

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis?

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms?

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes?

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases?

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases?

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders?

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.