Active Recombinant Human S100A8 protein, His-tagged

• Cat.No.: S100A8-2671H
• Product Overview: Active Recombinant Human S100A8 protein(NP_002955.2) (Leu 2-Glu 93) was expressed in E. coli, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 2-93 aa
• Form: Lyophilized from sterile 20mM Tris, pH 8.5, 10% glycerol.
• Bio-activity: 1. Measured by its ability to bind recombinant human S100A9 in a functional ELISA. 2. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-30 μg/mL.
• Molecular Mass: The recombinant human S100A8 consisting of 103 amino acids and migrates as an approximately 12.2 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_002955.2
• Unit ID: P05109
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P05109

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; CAGA, CFAG, S100 calcium binding protein A8 (calgranulin A), S100 calcium binding protein A8 (calgranulin A); protein S100-A8; 60B8AG; CGLA; MRP8; P8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); MIF; NIF; CAGA; CFAG; L1Ag; CP-10; MA387
• Gene ID: 6279
• mRNA Refseq: NM_002964
• protein Refseq: NP_002955
• MIM: 123885