Recombinant Rat S100A8 Protein

• Cat.No.: S100A8-5215R
• Product Overview: Recombinant Rat S100A8 full length or partial length protein was expressed.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: S100a8 S100 calcium binding protein A8 [ Rattus norvegicus ]
• Official Symbol: S100A8
• Gene ID: 116547
• mRNA Refseq: NM_053822.2
• Protein Refseq: NP_446274.2
• UniProt ID: P50115

Citation

Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction

Journal: Journal of Translational Medicine    PubMed ID: 26792056    Data: 2016/1/20

Authors: Roseanne Raphael, Diana Purushotham, Jennifer L. Strande

Article Snippet:Supplies and other reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless specified.Supplies and other reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless specified.. Recombinant S100A8 was purchased from Creative BioMart (Shirley, NY).

Proteins preferential to either HFpEF or control groups

Representative MS/MS scan for S100A8 peptide sequence ALNSIIDVYHK. Raw m/z spectral images with peak assignments and b and y ion lists along with a representation of peptide sequencing by tandem mass spectrometry

Plasma levels of S100A8 in control vs. HFpEF groups. a S100A8 is found in increased levels in the plasma of subjects with HFpEF vs. control subjects as detected by ELISA. The MCW columns include the control (n = 7) and HFpEF (n = 9) from the discovery cohort and the NWU colums include the control (n = 18) and HFpEF (n = 25) samples from the validation cohort. *p < 0.006 vs MCW Control. # p < 0. 002 vs NWU Control