|Description:||The TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha. The kit uses a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (ES2) in a homogeneous mix-and-read assay format.
The Lanthascreen ER alpha Competitive Binding Assay is:
• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs
|Applications:||Competitive Binding Assay, Nuclear Receptor Assay|
|Storage:||1 tube ER alpha Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
1 tube of Fluormone ES2 (store at -5 to -30°C)
2 bottles NR Buffer (store at -5 to -30°C)
1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
1 tube of DTT (store at -5 to -30°C)
|Size:||800 x 20 µL assays|
Price does not include shipping and packaging costs.Request quote for final price.