"TR-FRET" Related Products

TR-FRET ER alpha Competitive Binding Kit

Cat.No.: Kit-0973
Description: The TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha. The kit uses a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (ES2) in a homogeneous mix-and-read assay format.

The Lanthascreen ER alpha Competitive Binding Assay is:
• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs
Applications: Competitive Binding Assay, Nuclear Receptor Assay
Storage: 1 tube ER alpha Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
1 tube of Fluormone ES2 (store at -5 to -30°C)
2 bottles NR Buffer (store at -5 to -30°C)
1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
1 tube of DTT (store at -5 to -30°C)
Size: 800 x 20 µL assays
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