TR-FRET Glucocorticoid Receptor Competitive Binding Kit Kit-0953

Cat.No.:	Kit-0953
Description:	The TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (GS1) in a homogeneous mix-and-read assay format. The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is: • Complete & ready-to-use out of the box—all reagents included, just add your test compounds • High throughput compatible—TR-FRET assays in a 384-well plate • Convenient—components available in bulk sizes for larger screening needs
Applications:	Competitive Binding Assay, Nuclear Receptor Assay
Storage:	1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C) 1 tube of Fluormone GS1 (store at -5 to -30°C) 2 bottles Buffer (store at -5 to -30°C) 1 tube Tb-Anti-GST Ab (store at -5 to -30°C) 5 tubes GR Stabilizing Peptide (store at -5 to -30°C) 1 tube of DTT (store at -5 to -30°C)
Size:	800 x 20 µL assays

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