"TR-FRET" Related Products

TR-FRET Glucocorticoid Receptor Competitive Binding Kit

Cat.No.: Kit-0953
Description: The TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (GS1) in a homogeneous mix-and-read assay format.

The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs
Applications: Competitive Binding Assay, Nuclear Receptor Assay
Storage: 1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
1 tube of Fluormone GS1 (store at -5 to -30°C)
2 bottles Buffer (store at -5 to -30°C)
1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
5 tubes GR Stabilizing Peptide (store at -5 to -30°C)
1 tube of DTT (store at -5 to -30°C)
Size: 800 x 20 µL assays
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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