"TR-FRET" Related Products

      TR-FRET Glucocorticoid Receptor Competitive Binding Kit

      Cat.No. : Kit-0953
      Description : The TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (GS1) in a homogeneous mix-and-read assay format.

      The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is:

      • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
      • High throughput compatible—TR-FRET assays in a 384-well plate
      • Convenient—components available in bulk sizes for larger screening needs
      Applications : Competitive Binding Assay, Nuclear Receptor Assay
      Storage : 1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
      1 tube of Fluormone GS1 (store at -5 to -30°C)
      2 bottles Buffer (store at -5 to -30°C)
      1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
      5 tubes GR Stabilizing Peptide (store at -5 to -30°C)
      1 tube of DTT (store at -5 to -30°C)
      Size : 800 x 20 µL assays
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