TR-FRET Glucocorticoid Receptor Competitive Binding Kit

• Cat.No.: Kit-0953

Specification

• Tag: Non
• Description: The TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (GS1) in a homogeneous mix-and-read assay format.
  
  The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is:
  
  • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
  • High throughput compatible—TR-FRET assays in a 384-well plate
  • Convenient—components available in bulk sizes for larger screening needs
• Applications: Competitive Binding Assay, Nuclear Receptor Assay
• Storage: 1 tube GR Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
  1 tube of Fluormone GS1 (store at -5 to -30°C)
  2 bottles Buffer (store at -5 to -30°C)
  1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
  5 tubes GR Stabilizing Peptide (store at -5 to -30°C)
  1 tube of DTT (store at -5 to -30°C)
• Size: 800 x 20 µL assays