TR-FRET PPAR alpha Coactivator Assay Kit, rabbit
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Cat.No. : | Kit-0930 Optional Service: Optional requirements on this protein |
Description : | The TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. Agonist mode: When running the TR-FRET PPAR alpha Coactivator Assay in agonist mode (to identify agonist compounds), PPAR alpha- LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide. Antagonist mode: When the TR-FRET PPAR alpha Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR alpha-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-labeled anti-GST antibody. The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode. |
Applications : | Nuclear Receptor Assay |
Storage : | Tb-anti-GST Antibody (Rabbit): Store at -20 °C PPAR alpha-LBD, GST: Store at -80 °C Fluoroscein Coactivator Peptide: Store at -20 °C TR-FRET Coregulatore Buffer J: Store at 4 °C 1M DTT: Store at -20 °C or -80 °C |
Size : | 800 x 20 µL assays |
Easy access to products and services you need from our library via powerful searching tools.
Copyright © 2023 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy