"TR-FRET" Related Products

      TR-FRET RAR beta Coactivator Assay Kit, goat

      Cat.No. : Kit-0967
      Description : The TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

      When running the TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide.
      Applications : Co-Factor Interaction Assay
      Storage : Store in ultra-cold freezer (-68 to -85°C).
      Shipping : Dry Ice
      Size : 800 x 20 µL assays
      Kit Components : The TR-FRET RAR beta Coactivator Assay Kit contains RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
      Materials Required but Not Supplied : Microplate Reader
      Detection method : Fluorescent
      Products Types

      Online Inquiry

      Note: There will be extra charge for optional service!

      Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

      Optional requirements on this protein    +Expand

      Price Inquiry

      Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.