|Description:||The TR-FRET Retinoid X Receptor (RXR) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RXR beta ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide.
How it works
The TR-FRET RXR beta Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RXR beta ligand-binding domain (RXR beta-LBD) that is tagged with glutathione-S-transferase (GST). To assay, the RXR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520: 495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound
|Applications:||Co-Factor Interaction Assay|
|Storage:||Store in ultra-cold freezer (-68 to -85°C).|
|Size:||800 x 20 µL assays|
|Kit Components:||The TR-FRET RXR beta Coactivator Assay Kit contains RXR beta-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).|
|Materials Required but Not Supplied:||Microplate Reader|